The Institute proposes “Blastocyst culture” for infertile patient. Blastocyst culture is the most advance infertility treatment which involves in vitro fertilization and embryo culture for 5 to 6 days until it reaches the blastocyst stage – a final stage of embryo that is ready to implant in to the uterine wall and become pregnancy. The best embryos are selected and transfer into the uterine cavity after they have been analyzed for any chromosome abnormalities to ensure of having normal chromosome baby before implanting it into the uterus. Patients with tubal blockage, severe pelvic adhesion, endometriosis, male factor problem and unexplained infertility are good candidates for this treatment.
Blastocyst Culture Modern Technology for Infertility
What is Blastocyst ?
Blastocyst is a 5 days old embryo that is formed after the conception of the egg and the sperm. The embryo size before being embedded is 120-150 cells. The embryo cell is divided into 2 types: a group of cells that develop into a fetus and a group of cells that is formed outside the placenta. This is the only cell that is different from the embryo during the cleaving stage (stage: 1-16 cells) that is growing less than 5 days.
Blastocyst Culture is the latest version of fertilization method of conceiving the egg and the sperm outside the body and rearing the embryo until it reaches the blastocyst stage. During the blastocyst, the embryo is implanted into the intrauterine and raised in the fetus. It takes 5 days to rear the embryo outside the body until the blastocyst stage. This method is different from the traditional baby tube fertilization where the embryo is implanted into the intrauterine during the cleaving stage (only 4-8 cells) by rearing the embryo externally for only 2-3 days.
Today, advanced environmental development has helped to prolong the external embryo rearing to 5-6 days until the embryo reaches the stage where it can be implanted as an infant in the blastocyst stage. This is the stage where the embryo can be immediately implanted into the intrauterine, which results for a better chance of pregnancy, rather than implanting the embryo at other period of the embryo stage.
•Stimulate the egg to produce as much ovaries as possible by using injection. One injection is continuously injected daily for 7-10 days. Normally, 8-10 ovaries are required. Currently, more purified stimulant is commonly used to produce ovaries, reducing the amount of injection and medicine
•Once the egg is stimulated, the doctor would follow-up the status of the egg growth by using ultrasound. When the egg is fully developed, the doctor would perforate the egg by absorbing the egg cell externally from the body
•Egg perforation is done through the vagina by using the ultrasound to indicate the position of the fetus. A small needle is used to perforate the egg. It takes 5-10 minutes only. The doctor uses suppressant during the perforation. The patient can go home after the treatment
•The semen is stored in a container. A special equipment and pure sperm liquid are used to select the efficient, germ-free and strongest sperm before conceiving with the egg
•The egg and sperm are conceived. The egg is incubated in the testing room for 5 days
•During the blastocyst stage, the embryo is implanted into the intrauterine by inserting a small, plastic tube into the vagina. The patient is fully aware of the insertion process without feeling any pain. The patient is required to rest for at least 2 hours before returning home
•A periodic appointment is required to track the level of hormone balance and to test is the embryo implantation is suitable for the intrauterine membrane
•After 14 days of the embryo implantation, the doctor would test he pregnancy by perforating for hCG hormone or the placenta hormone
Advantages of Blastocyst Culture
•The pregnancy tendency rate is high
•Selection of good quality embryo is used for the best growth in the intrauterine
•Blastocyst is a method where the embryo that can be naturally implanted in the intrauterine
•Reduce the risk of having more than 2 twins since the ratio of pregnancy is higher than any other methods used, even though 1-2 embryos are implanted
•Ability to diagnose the embryo abnormality before implanting the embryo into the intrauterine (PGD)
•No pain, no operation or no stomach perforation is required
•No hospital admission is required
Testicular Sperm Extraction (TESE)
A method where the semen is extracted from the testicles for cases where the male is unable to produce sperm from the semen excreted. The semen tube may be clogged or partially cut off (sterile). TESE is a minor and uncomplicated operation. A part of the tissue is cut off from the testicles by using a needle to absorb the sperm for identification. Anesthetic or anti-suppressant are used for the restricted operation area. The tested sperm is injected into the egg by ICSI process.
Intra Cytoplasmic Sperm Injection (ICSI)
This technique is a direct injection of a single sperm into each egg. This is one way of conception for patients who have a few sperm or no sperm to penetrate into the egg by itself.
Perfect Woman Institute
2123/1 New Petchburi Road, Bangkapi, Huaykwang,
Bangkok 10310 Thailand